Headerimage Platform A. Technology

Platform A. Technology

Platform A: Techniques and Technology Development

Platform Coordinators

Prof. Dr. Ralph P. Brandes

Prof. Dr. Ralf P. Brandes

  • Institute for Cardiovascular Physiology
  • Goethe-University Frankfurt
    Theodor-Stern-Kai 7
    60590 Frankfurt/Main
  • Write E-Mail
Prof. Dr. Ralph T. Schermuly

Prof. Dr. Ralph T. Schermuly

  • Pulmonary Pharmacotherapy
  • Justus-Liebig-University Giessen
    Aulweg 130
    35392 Giessen
  • Write E-Mail
Prof. Dr. Dr. Thomas Braun

Prof. Dr. Dr. Thomas Braun

  • Max-Planck-Institute for Heart- and Lung Research
    Ludwigstr. 43
    61231 Bad Nauheim
  • Write E-Mail

The ECCPS provides a broad spectrum of technologies for the analysis of the cardio-pulmonary system, ranging from techniques to study single molecules through pre-clinical phenotyping models to human-related technology e.g. biomarkers, biobanks and interventional trials.

Service units:

  • Bioinformatics and deep sequencing (service oriented bioinformatics facility, deep sequencing analysis platform, integration of "omics" technologies)
  • Cardio-pulmonary deep phenotyping (stress test and mouse disease facility, small animal imaging platform)
  • Molecular analysis and high resolution imaging (molecular in vivo imaging, molecular analytic platform)

 Technologies currently available in the ECCPS

Level Techniques
Genomics, Transcriptomics cDNA microarray platform including bioinformatics, chip manufacturing, DNA sequencing,SNPs, STRs, chromatin immunoprecipitation (ChIP), two/three-hybrid screens, microarraysfor protein-DNA interaction, RNA fingerprinting /RAP-PCR, SNP pyrosequencing laser-mediatedmicro dissection, DNA methylation assay
Proteomics MALDI-TOF/TOF-MS, ESI-MS/MS, SELDI-MS, Nano-LC-ESI-MS/MS, protein profiling onantibody chip, DNA-assisted protein chip, ELISPOT, micro 2-D protein-analysis, tissuemicroassay, peptide mapping & sequencing, cross-linking analysis, phosphoproteome, phage-display screen, surface plasmon resonance
Lipidomics, Glyconomics HPTLC, ESI-MS, LC-MS/MS, 2D-HPLC, GC/MS, MALDI-TOF/TOF-MS, nano-ESI-MS/MS, carbohydrate composition & linkage analysis, LC-MS/MS
Protein Biochemistry Isothermal titration calorimetry, fluorescence spectroscopy, fluorescence resonance energy transfer between site-specific fluorescently labelled macromolecules, circular dichroism, cross linking of site-specific chemically modified macromolecules, fluorescence stopped flow, quenched flow, SPOT analysis, chromatography units
ROS/NO Detection ESR spectroscopy, in-situ remission spectroscopy, chemiluminescence, single cell microfluorometry, respiratory measurements
Imaging - Molecular FRET, BRET, FRAP, confocal microscopy, electron microscopy, automated high throughput in situ hybridization, in-situ PCR, immunocyto(histo)chemistry, cell-edge-detection system for determination of contractile function, cell coupling; fluorimetric second messenger detection, activatable smart probes, live cell imaging
Imaging - Small animal Small animal NMR, micro-CT, flat-panel volumetric CT, mouse echocardiography, hybrid fluorescence molecular tomography-CT
Imaging - Morphometry Software-assisted vascular morphometry (medial wall thickness, lumen area, degree of muscularization), alveolar morphometric routines (septal wall thickness, mean alveolar intersept & airspace), heart stereology, assessment of innervation
Imaging - Clinical Cardiac MRT, IVUS, MS CT, virtual imaging, 3-D echocardiography
Cytometric Profiling FACS-based multiparametric immunophenotyping/sorting , high volume immunomagnetic cell sorting (autoMACS); analysis of cell-specific gene & protein expression (multicolor fluorescent antibodies) in complex cell samples, ELISPOT
Transfection Technology Adeno-, retro-virus & bacteria mediated gene transfer, liposome based gene transfer technologies, PEI-DNA aerosol technology, aerosolized nanocarriers for gene transfer, ribozymes, antisense technology, VP22-mediated protein-transfer
Gene Silencing siRNA manufacturing, siRNA aerosolization & vector technology, conditional RNAi in mice (Tet control, Cre/lox control), lentiviral siRNA transfer
Stem Cell Technologies Large scale murine ES-cell production induced ES-cell differentiation and targeted manipulation of cell development. Purification of human & murine progenitors, circulating cells & (cancer-) stem cells. In vivo tracing of stem cell progeny with reporter-transgenes, TK constructs, & radioactive labelling, GMP facilities & permission for the isolation & cultivation of human stem cells for clinical use (production license HA-FE-504/A)
Angiogenesis Models In-vitro assays (Fibrin & Matrigel assays), Matrigel plug assays in mice, hind limb ischemia model, tumour angiogenesis models, ES cell angiogenesis assay
Transgenic Mouse Facility ES cell bank, generation of transgenic mice, knock-out/ -in mutants, of cell-type-specific temporally-controlled somatic mutants, direct links to several mouse consortia (IKMC, EUCOMM, GGTC, DiGtoP, NGFNplus, EMMA, NGFNplus)
Mouse Phenotyping Invasive, non-invasive & telemetric hemodynamic assessment, echocardiography, mouse lung & airway (bronchoconstriction, mucociliary clearance) function testing, precision cut lung slices
Mouse Models of Disease Cardiac ischemia & infarction, left & right heart hypertrophy & failure, atherosclerosis, restenosis, allograft vasculopathy, pulmonary hypertension, lung fibrosis, pulmonary emphysema / COPD, ARDS, ventilator-induced lung injury, bacterial and viral pneumonia, emphysema, sleep apnoea, lung tumour & metastasis, thrombosis models
Zebrafish & C. elegans Breeding, transgenic fish, models of cardiac & vascular development, manipulation, generation & characterization of C. elegans